Review



murine period 2 per2 promoter  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc murine period 2 per2 promoter
    ( A ) Differentiation protocol of miPSCs to chondrocytes. miPSCs were subjected to high-density micromass culture to create PDiPSCs. PDiPSCs were then either cast in agarose or pelleted and cultured in chondrogenic media for 14 to 21 days to create tissue-engineered cartilage. ( B ) P2L bioluminescence intensity (BLI) of miPSCs ( n = 10), PDiPSCs ( n = 2), PDiPSCs in agarose ( n = 4), and pellets ( n = 10) (left) and B1L bioluminescence intensity (BLI) of miPSCs ( n = 5), PDiPSCs ( n = 4), PDiPSCs in agarose ( n = 5) (middle), and pellets ( n = 5) (right). ( C ) BLI of femoral head cartilage explants from <t>PER2::LUC</t> mice ( n = 3, one hip per mouse). ( D ) P2L BLI in porcine chondrocytes cast in agarose ( n = 5). Shaded region on graphs represents SEM.
    Murine Period 2 Per2 Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+period+2+per2+promoter/pmc09132444-152-20-25?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    murine period 2 per2 promoter - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1–induced inflammation"

    Article Title: Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1–induced inflammation

    Journal: Science Advances

    doi: 10.1126/sciadv.abj8892

    ( A ) Differentiation protocol of miPSCs to chondrocytes. miPSCs were subjected to high-density micromass culture to create PDiPSCs. PDiPSCs were then either cast in agarose or pelleted and cultured in chondrogenic media for 14 to 21 days to create tissue-engineered cartilage. ( B ) P2L bioluminescence intensity (BLI) of miPSCs ( n = 10), PDiPSCs ( n = 2), PDiPSCs in agarose ( n = 4), and pellets ( n = 10) (left) and B1L bioluminescence intensity (BLI) of miPSCs ( n = 5), PDiPSCs ( n = 4), PDiPSCs in agarose ( n = 5) (middle), and pellets ( n = 5) (right). ( C ) BLI of femoral head cartilage explants from PER2::LUC mice ( n = 3, one hip per mouse). ( D ) P2L BLI in porcine chondrocytes cast in agarose ( n = 5). Shaded region on graphs represents SEM.
    Figure Legend Snippet: ( A ) Differentiation protocol of miPSCs to chondrocytes. miPSCs were subjected to high-density micromass culture to create PDiPSCs. PDiPSCs were then either cast in agarose or pelleted and cultured in chondrogenic media for 14 to 21 days to create tissue-engineered cartilage. ( B ) P2L bioluminescence intensity (BLI) of miPSCs ( n = 10), PDiPSCs ( n = 2), PDiPSCs in agarose ( n = 4), and pellets ( n = 10) (left) and B1L bioluminescence intensity (BLI) of miPSCs ( n = 5), PDiPSCs ( n = 4), PDiPSCs in agarose ( n = 5) (middle), and pellets ( n = 5) (right). ( C ) BLI of femoral head cartilage explants from PER2::LUC mice ( n = 3, one hip per mouse). ( D ) P2L BLI in porcine chondrocytes cast in agarose ( n = 5). Shaded region on graphs represents SEM.

    Techniques Used: Cell Culture

    Per2 expression in cartilage explants from the femoral head of PER2::LUC mice was recorded as bioluminescence intensity. Explants were treated with IL-1β (1 ng/ml), IL-1⍺ (1 ng/ml), TNF⍺ (20 ng/ml), or vehicle controls ( n = 3 per each condition). Loss of circadian rhythm was seen in explants, given IL-1 and circadian rhythm was rescued with the administration of dex. Gray, blue, orange, and red bars indicate administration of PBS or cytokine; black bars indicate administration of dex. Shaded region on graph represents SEM. Asterisks represent significance compared to controls ( P < 0.05). ** P < 0.01; *** P < 0.05.
    Figure Legend Snippet: Per2 expression in cartilage explants from the femoral head of PER2::LUC mice was recorded as bioluminescence intensity. Explants were treated with IL-1β (1 ng/ml), IL-1⍺ (1 ng/ml), TNF⍺ (20 ng/ml), or vehicle controls ( n = 3 per each condition). Loss of circadian rhythm was seen in explants, given IL-1 and circadian rhythm was rescued with the administration of dex. Gray, blue, orange, and red bars indicate administration of PBS or cytokine; black bars indicate administration of dex. Shaded region on graph represents SEM. Asterisks represent significance compared to controls ( P < 0.05). ** P < 0.01; *** P < 0.05.

    Techniques Used: Expressing



    Similar Products

    murine period 2 per2 promoter  (Addgene inc)
    93
    Addgene inc murine period 2 per2 promoter
    ( A ) Differentiation protocol of miPSCs to chondrocytes. miPSCs were subjected to high-density micromass culture to create PDiPSCs. PDiPSCs were then either cast in agarose or pelleted and cultured in chondrogenic media for 14 to 21 days to create tissue-engineered cartilage. ( B ) P2L bioluminescence intensity (BLI) of miPSCs ( n = 10), PDiPSCs ( n = 2), PDiPSCs in agarose ( n = 4), and pellets ( n = 10) (left) and B1L bioluminescence intensity (BLI) of miPSCs ( n = 5), PDiPSCs ( n = 4), PDiPSCs in agarose ( n = 5) (middle), and pellets ( n = 5) (right). ( C ) BLI of femoral head cartilage explants from <t>PER2::LUC</t> mice ( n = 3, one hip per mouse). ( D ) P2L BLI in porcine chondrocytes cast in agarose ( n = 5). Shaded region on graphs represents SEM.
    Murine Period 2 Per2 Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+period+2+per2+promoter/pmc09132444-152-20-25?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    murine period 2 per2 promoter - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Differentiation protocol of miPSCs to chondrocytes. miPSCs were subjected to high-density micromass culture to create PDiPSCs. PDiPSCs were then either cast in agarose or pelleted and cultured in chondrogenic media for 14 to 21 days to create tissue-engineered cartilage. ( B ) P2L bioluminescence intensity (BLI) of miPSCs ( n = 10), PDiPSCs ( n = 2), PDiPSCs in agarose ( n = 4), and pellets ( n = 10) (left) and B1L bioluminescence intensity (BLI) of miPSCs ( n = 5), PDiPSCs ( n = 4), PDiPSCs in agarose ( n = 5) (middle), and pellets ( n = 5) (right). ( C ) BLI of femoral head cartilage explants from PER2::LUC mice ( n = 3, one hip per mouse). ( D ) P2L BLI in porcine chondrocytes cast in agarose ( n = 5). Shaded region on graphs represents SEM.

    Journal: Science Advances

    Article Title: Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1–induced inflammation

    doi: 10.1126/sciadv.abj8892

    Figure Lengend Snippet: ( A ) Differentiation protocol of miPSCs to chondrocytes. miPSCs were subjected to high-density micromass culture to create PDiPSCs. PDiPSCs were then either cast in agarose or pelleted and cultured in chondrogenic media for 14 to 21 days to create tissue-engineered cartilage. ( B ) P2L bioluminescence intensity (BLI) of miPSCs ( n = 10), PDiPSCs ( n = 2), PDiPSCs in agarose ( n = 4), and pellets ( n = 10) (left) and B1L bioluminescence intensity (BLI) of miPSCs ( n = 5), PDiPSCs ( n = 4), PDiPSCs in agarose ( n = 5) (middle), and pellets ( n = 5) (right). ( C ) BLI of femoral head cartilage explants from PER2::LUC mice ( n = 3, one hip per mouse). ( D ) P2L BLI in porcine chondrocytes cast in agarose ( n = 5). Shaded region on graphs represents SEM.

    Article Snippet: Two luciferase reporter lentiviral vectors, one driven by the murine Bmal1 promoter (Addgene no. 182761) and one driven by the murine Period 2 (Per2) promoter (Addgene no. 182762), were used ( ).

    Techniques: Cell Culture

    Per2 expression in cartilage explants from the femoral head of PER2::LUC mice was recorded as bioluminescence intensity. Explants were treated with IL-1β (1 ng/ml), IL-1⍺ (1 ng/ml), TNF⍺ (20 ng/ml), or vehicle controls ( n = 3 per each condition). Loss of circadian rhythm was seen in explants, given IL-1 and circadian rhythm was rescued with the administration of dex. Gray, blue, orange, and red bars indicate administration of PBS or cytokine; black bars indicate administration of dex. Shaded region on graph represents SEM. Asterisks represent significance compared to controls ( P < 0.05). ** P < 0.01; *** P < 0.05.

    Journal: Science Advances

    Article Title: Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1–induced inflammation

    doi: 10.1126/sciadv.abj8892

    Figure Lengend Snippet: Per2 expression in cartilage explants from the femoral head of PER2::LUC mice was recorded as bioluminescence intensity. Explants were treated with IL-1β (1 ng/ml), IL-1⍺ (1 ng/ml), TNF⍺ (20 ng/ml), or vehicle controls ( n = 3 per each condition). Loss of circadian rhythm was seen in explants, given IL-1 and circadian rhythm was rescued with the administration of dex. Gray, blue, orange, and red bars indicate administration of PBS or cytokine; black bars indicate administration of dex. Shaded region on graph represents SEM. Asterisks represent significance compared to controls ( P < 0.05). ** P < 0.01; *** P < 0.05.

    Article Snippet: Two luciferase reporter lentiviral vectors, one driven by the murine Bmal1 promoter (Addgene no. 182761) and one driven by the murine Period 2 (Per2) promoter (Addgene no. 182762), were used ( ).

    Techniques: Expressing